Project Highlights

NextDx prototype

NextDx prototype cartridge with waveguide on a prototype analyzer built at Philips.

Biophysical technique for binding discrimination

A biophysical measurement technique has been developed to study binding discrimination. The technique is based on the detection of thermal motion of magnetic particles. Analysis of a sequence of recorded images yields position distribution patterns of individual particles (left). Multiple particles are tracked in parallel (right) and are analyzed on binding type.
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Optical detection of particle labels

Concept of the waveguide based detection system in the FP7 NextDx project: the evanescent tail of the light propagating in the waveguide excites only particles close to the surface, which can be imaged using dark field detection. More info:

Nano-signal generation

The optical response is studied for different particle parameters (the size, the refractive index, the roughness) and for different parameters of the optical system (the waveguide material, the wavelength). The Null-Field Method with Discrete Sources (NFM-DS) has been set up to compute the light scattering (total cross-section, dark-field) by a magnetic bead on a planar waveguide.

Development of novel capture molecules

Epitope mapping of HyTest’s monoclonal antibodies specific to human cardiac Troponin I. The best of these antibodies are in the process of development of novel capture molecules. For this purpose a set of recombinant scFv or Fab fragments of monoclonal antibodies was successfully produced by HyTest.

Polymer slab waveguides

Design and fabrication of polymer slab waveguides for the optical detection of nanoparticles in evanescent field based biosensors. (left) cross-section of a single mode slab waveguide and (right) near-field profile at the end-face of the slab waveguide (arbitrary units). More info: